Phosphorus-containing macromolecular assemblies in the cell nuclei of drosophila larvae

Image of phosphorus-containing macromolecular assemblies in the cell nuclei of drosophila larvae; dual-axis EFTEM tilt series acquired with a Tridiem energy filter from -66˚ to 66˚; for each axis phosphorus L-23 series (20 eV slit, pre-edge and post-edge) were collected; a jump-ratio series was calculated and the data were aligned and reconstructed using IMOD (University of Boulder, Colorado); finally, the reconstruction was rendered in the 3D visualization software; the inset highlights a sub-volume extracted using the 3D visualization ROI tool.